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Thesis Format for San Pablo catholic university (UCSP), computer science program.
Formato de Tésis de la universidad católica San Pablo para el programa de ciencia de la computación
José Manuel Valdivia Romero, Adolfo Tamayo Briceño, Ernesto Cuadros Vargas
Bacterial cells can have DNA damage due to transcriptional error, or through the effect of an antibiotic. The SOS response is a bacterial cell program for coping with DNA damage, in which the cell cycle is arrested, and DNA repair is induced. The repairs have high probability in leading to mutagenesis in the bacteria, which can lead to antibiotic resistance. The RecA protein in bacteria is responsible for the activation of the SOS response; therefore, making it a target for inhibition. I elected to use the ubiquitination system, natively used for apoptosis, as a means of targeted degradation of the RecA protein in bacteria prone to mutations. Polyubiquitination of misfolded proteins leads to the breaking down of the protein with the aid of proteasomes, which break down unnecessary proteins through a chemical reaction known as proteolysis. Using random forest-predictors, I determined a statistically high likelihood of ubiquitination of the RecA protein in MRSA, Tuberculosis, and other high risk bacterial infections. I hypothesized that I could foster ubiquitin-tagging on RecA by forcing the protein to misfold. Chaperones are proteins which interact with each other to prevent specific sets of proteins from misfolding. CHIP (C terminus of HSC70-Interacting Protein) is a biomolecule that inhibits interactions between the chaperones of RecA. Adding CHIP, ubiquitin, and 26s proteasomes into the bacterial system, should theoretically lead to the degradation of the RecA protein inside the bacteria. I tested my hypothesis by conducting an assay for monitoring CHIP-mediated ubiquitination, and conducted analysis on the assay using SDS- Page gel electrophoresis, and Western-blotting. The resulting data showed signs of polyubiquitination on the RecA protein, with chains of five or more ubiquitin, showing high drug potential. Adding an antibody drug conjugate, containing all the necessary components of a CHIP-mediated ubiquitination reaction, to common antibiotics can lead to the inhibition of bacterial mutagenesis, and higher antibiotic drug potency.
An experiment investigating full wave rectification, for the purposes of producing a steady DC output. This is implemented using every half-cycle input from the supply voltage instead of every other half, this is known as a full wave rectifier.
Una plantilla atractiva, moderna y dinámica para evaluaciones y guías de la UC.
Esta plantilla es una derivación directa de la plantilla existente y públicamente liberada de la Escuela de Ingeniería de la Universidad de Edinburgh. Puedes ver mas información de la licencia pertinente en LICENSE.md
La plantilla otorga diversas opciones que permiten formar un documento listo en minutos, con alta flexibilidad y código mantenible.
La plantilla otorga diversas opciones que permiten formar un documento listo en minutos, con alta flexibilidad y código mantenible, entre otras:
Soporte de emojis ✨
Soporte de bloques de código (con `formato`) ⌨️
Unidad Académica
Código del Curso y Título
Fecha del Documento
Presencia de respuestas (genera rúbricas automáticamente)
Número de preguntas que hay que responder
Si se puede utilizar calculadora
Si se incluye una hoja de fórmulas
Si la prueba es con libro abierto
Si requiere puntaje por pregunta
...
Se puede encontrar mas información y documentación sobre el proyecto en agucova.github.io/plantilla-uc
Agustín Covarrubias (derived from F. Teixeira-Dias, A. Giannopoulos)
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